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cd45 fitc antibody  (Miltenyi Biotec)


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    Miltenyi Biotec cd45 fitc antibody
    Cd45 Fitc Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 307 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45+fitc/CD45+Antibody%2C+anti-human%2C+REAfinity/pmc13373028-168-68-71
    Average 96 stars, based on 307 article reviews
    cd45 fitc antibody - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Influence of Donor Age, Donor Body Mass Index, and Harvesting Site on Cell Preparations from Human Adipose Tissue
    Article Snippet: .. For analysis of the composition of the SVF, 1 × 10 6 total cells were filled up with autoMACS running buffer (Miltenyi Biotech, Bergisch Gladbach, Germany) to 5 mL and centrifuged for 5 min at RT at 400× g . The cells were incubated with blocking buffer (Human Tandem Signal Enhancer, Miltenyi Biotech) for 10 min. on ice to prevent nonspecific binding of fluorophores, followed by an additional 20 min. incubation with CD31-BV605 (1:20, BioLegend, San Diego, CA, USA), CD34-PE (1:50, BioLegend), CD45-FITC (1:50, Miltenyi), CD90-APC (1:20, Biozol, Eching, Germany), CD144-PE-Vio700 (1:20, Miltenyi), and CD146-BV711 (1:20, BioLegend). ..

    Article Title: Influence of Donor Age, Donor Body Mass Index, and Harvesting Site on Cell Preparations from Human Adipose Tissue
    Article Snippet: .. For analysis of the composition of the SVF, 1 × 106 total cells were filled up with autoMACS running buffer (Miltenyi Biotech, Bergisch Gladbach, Germany) to 5 mL and centrifuged for 5 min at RT at 400× g. The cells were incubated with blocking buffer (Human Tandem Signal Enhancer, Miltenyi Biotech) for 10 min. on ice to prevent nonspecific binding of fluorophores, followed by an additional 20 min. incubation with CD31-BV605 (1:20, BioLegend, San Diego, CA, USA), CD34-PE (1:50, BioLegend), CD45-FITC (1:50, Miltenyi), CD90-APC (1:20, Biozol, Eching, Germany), CD144-PE-Vio700 (1:20, Miltenyi), and CD146-BV711 (1:20, BioLegend). ..

    Blocking Assay:

    Article Title: Influence of Donor Age, Donor Body Mass Index, and Harvesting Site on Cell Preparations from Human Adipose Tissue
    Article Snippet: .. For analysis of the composition of the SVF, 1 × 10 6 total cells were filled up with autoMACS running buffer (Miltenyi Biotech, Bergisch Gladbach, Germany) to 5 mL and centrifuged for 5 min at RT at 400× g . The cells were incubated with blocking buffer (Human Tandem Signal Enhancer, Miltenyi Biotech) for 10 min. on ice to prevent nonspecific binding of fluorophores, followed by an additional 20 min. incubation with CD31-BV605 (1:20, BioLegend, San Diego, CA, USA), CD34-PE (1:50, BioLegend), CD45-FITC (1:50, Miltenyi), CD90-APC (1:20, Biozol, Eching, Germany), CD144-PE-Vio700 (1:20, Miltenyi), and CD146-BV711 (1:20, BioLegend). ..

    Article Title: ΔNp73 isoform defines a TP53 -mutant-like poor-risk subgroup of acute myeloid leukemia
    Article Snippet: .. To analyze the hematopoietic stem progenitor cell (HSPC) populations of the AML bulk samples, 5x10 5 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained with the following antibodies: CD45-FITC, CD34-PE, CD38-BV421, CD11b-PECy7, CD14 PerCP, Sca-1-APC (to exclude MS5 cells) and viability marker 7-AAD for 20 min at 4°C. .. Fluorescence was measured on the BD LSRII or FACS Symphony A5 and analyzed using Flow Jo (Tree Star, Inc).

    Article Title: ΔNp73 isoform defines a TP53-mutant-like poor-risk subgroup of acute myeloid leukemia.
    Article Snippet: .. To analyze the hematopoietic stem progenitor cell (HSPC) populations of the AML bulk samples, 5x105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained with the following antibodies: CD45-FITC, CD34-PE, CD38-BV421, CD11b-PECy7, CD14 PerCP, Sca-1-APC (to exclude MS5 cells) and viability marker 7-AAD for 20 min at 4◦C. .. Fluorescence was measured on the BD LSRII or FACS Symphony A5 and analyzed using Flow Jo (Tree Star, Inc).

    Article Title: Influence of Donor Age, Donor Body Mass Index, and Harvesting Site on Cell Preparations from Human Adipose Tissue
    Article Snippet: .. For analysis of the composition of the SVF, 1 × 106 total cells were filled up with autoMACS running buffer (Miltenyi Biotech, Bergisch Gladbach, Germany) to 5 mL and centrifuged for 5 min at RT at 400× g. The cells were incubated with blocking buffer (Human Tandem Signal Enhancer, Miltenyi Biotech) for 10 min. on ice to prevent nonspecific binding of fluorophores, followed by an additional 20 min. incubation with CD31-BV605 (1:20, BioLegend, San Diego, CA, USA), CD34-PE (1:50, BioLegend), CD45-FITC (1:50, Miltenyi), CD90-APC (1:20, Biozol, Eching, Germany), CD144-PE-Vio700 (1:20, Miltenyi), and CD146-BV711 (1:20, BioLegend). ..

    Binding Assay:

    Article Title: Influence of Donor Age, Donor Body Mass Index, and Harvesting Site on Cell Preparations from Human Adipose Tissue
    Article Snippet: .. For analysis of the composition of the SVF, 1 × 10 6 total cells were filled up with autoMACS running buffer (Miltenyi Biotech, Bergisch Gladbach, Germany) to 5 mL and centrifuged for 5 min at RT at 400× g . The cells were incubated with blocking buffer (Human Tandem Signal Enhancer, Miltenyi Biotech) for 10 min. on ice to prevent nonspecific binding of fluorophores, followed by an additional 20 min. incubation with CD31-BV605 (1:20, BioLegend, San Diego, CA, USA), CD34-PE (1:50, BioLegend), CD45-FITC (1:50, Miltenyi), CD90-APC (1:20, Biozol, Eching, Germany), CD144-PE-Vio700 (1:20, Miltenyi), and CD146-BV711 (1:20, BioLegend). ..

    Article Title: Influence of Donor Age, Donor Body Mass Index, and Harvesting Site on Cell Preparations from Human Adipose Tissue
    Article Snippet: .. For analysis of the composition of the SVF, 1 × 106 total cells were filled up with autoMACS running buffer (Miltenyi Biotech, Bergisch Gladbach, Germany) to 5 mL and centrifuged for 5 min at RT at 400× g. The cells were incubated with blocking buffer (Human Tandem Signal Enhancer, Miltenyi Biotech) for 10 min. on ice to prevent nonspecific binding of fluorophores, followed by an additional 20 min. incubation with CD31-BV605 (1:20, BioLegend, San Diego, CA, USA), CD34-PE (1:50, BioLegend), CD45-FITC (1:50, Miltenyi), CD90-APC (1:20, Biozol, Eching, Germany), CD144-PE-Vio700 (1:20, Miltenyi), and CD146-BV711 (1:20, BioLegend). ..

    Staining:

    Article Title: ΔNp73 isoform defines a TP53 -mutant-like poor-risk subgroup of acute myeloid leukemia
    Article Snippet: .. To analyze the hematopoietic stem progenitor cell (HSPC) populations of the AML bulk samples, 5x10 5 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained with the following antibodies: CD45-FITC, CD34-PE, CD38-BV421, CD11b-PECy7, CD14 PerCP, Sca-1-APC (to exclude MS5 cells) and viability marker 7-AAD for 20 min at 4°C. .. Fluorescence was measured on the BD LSRII or FACS Symphony A5 and analyzed using Flow Jo (Tree Star, Inc).

    Article Title: ΔNp73 isoform defines a TP53-mutant-like poor-risk subgroup of acute myeloid leukemia.
    Article Snippet: .. To analyze the hematopoietic stem progenitor cell (HSPC) populations of the AML bulk samples, 5x105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained with the following antibodies: CD45-FITC, CD34-PE, CD38-BV421, CD11b-PECy7, CD14 PerCP, Sca-1-APC (to exclude MS5 cells) and viability marker 7-AAD for 20 min at 4◦C. .. Fluorescence was measured on the BD LSRII or FACS Symphony A5 and analyzed using Flow Jo (Tree Star, Inc).

    Article Title: Preserved Function of Endothelial Colony-Forming Cells in Female Rats with Intrauterine Growth Restriction: Protection Against Arterial Hypertension and Arterial Stiffness?
    Article Snippet: .. Single-cell suspensions of ECFCs from CTRL (n = 4) and IUGR (n = 4) rats were stained with fluorochrome-labeled monoclonal antibodies against CD31 PE (TLD-3A12), CD45 FITC (OX-1), and CD146 APC (LSEC) (BD Biosciences, San Jose, CA, USA or Miltenyi Biotech, Bergisch Gladbach, Germany) in phosphate-buffered saline (PBS; CHUV) containing 3% fetal calf serum (FCS) for 20 min at 4 ◦C. .. After removal of unbound antibodies https://doi.org/10.3390/cells15020171 by centrifugation, cells were resuspended in 200 μL of PBS/3%FCS, and 0.5 μg of 4′,6- diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was added to identify dead cells.

    Article Title: Therapeutic efficacy by pulmonary delivery of live attenuated mycobacteria
    Article Snippet: Flow Cytometry Analysis 106 lung or BAL cells were incubated for 15 minutes at 4° C. with Fc receptor blocking reagent (Miltenyi Biotech). .. Then, eosinophil, neutrophil and macrophage presence was determined by extracellular staining with the following antibodies: CD45-FITC, siglecF-APC, Ly-6G-Vioblue®, from Miltenyi Biotech; and CD11c-PE, CD11b-PerCP/Cy5.5, from BD Biosciences. ..

    Article Title: Preserved Function of Endothelial Colony-Forming Cells in Female Rats with Intrauterine Growth Restriction: Protection Against Arterial Hypertension and Arterial Stiffness?
    Article Snippet: .. Single-cell suspensions of ECFCs from CTRL (n = 4) and IUGR (n = 4) rats were stained with fluorochrome-labeled monoclonal antibodies against CD31 PE (TLD-3A12), CD45 FITC (OX-1), and CD146 APC (LSEC) (BD Biosciences, San Jose, CA, USA or Miltenyi Biotech, Bergisch Gladbach, Germany) in phosphate-buffered saline (PBS; CHUV) containing 3% fetal calf serum (FCS) for 20 min at 4 °C. .. After removal of unbound antibodies by centrifugation, cells were resuspended in 200 μL of PBS/3%FCS, and 0.5 μg of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was added to identify dead cells.

    Marker:

    Article Title: ΔNp73 isoform defines a TP53 -mutant-like poor-risk subgroup of acute myeloid leukemia
    Article Snippet: .. To analyze the hematopoietic stem progenitor cell (HSPC) populations of the AML bulk samples, 5x10 5 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained with the following antibodies: CD45-FITC, CD34-PE, CD38-BV421, CD11b-PECy7, CD14 PerCP, Sca-1-APC (to exclude MS5 cells) and viability marker 7-AAD for 20 min at 4°C. .. Fluorescence was measured on the BD LSRII or FACS Symphony A5 and analyzed using Flow Jo (Tree Star, Inc).

    Article Title: ΔNp73 isoform defines a TP53-mutant-like poor-risk subgroup of acute myeloid leukemia.
    Article Snippet: .. To analyze the hematopoietic stem progenitor cell (HSPC) populations of the AML bulk samples, 5x105 mononuclear cells were blocked with human FcR blocking reagent (Miltenyi Biotec) for 5 min and stained with the following antibodies: CD45-FITC, CD34-PE, CD38-BV421, CD11b-PECy7, CD14 PerCP, Sca-1-APC (to exclude MS5 cells) and viability marker 7-AAD for 20 min at 4◦C. .. Fluorescence was measured on the BD LSRII or FACS Symphony A5 and analyzed using Flow Jo (Tree Star, Inc).

    Single Cell:

    Article Title: Preserved Function of Endothelial Colony-Forming Cells in Female Rats with Intrauterine Growth Restriction: Protection Against Arterial Hypertension and Arterial Stiffness?
    Article Snippet: .. Single-cell suspensions of ECFCs from CTRL (n = 4) and IUGR (n = 4) rats were stained with fluorochrome-labeled monoclonal antibodies against CD31 PE (TLD-3A12), CD45 FITC (OX-1), and CD146 APC (LSEC) (BD Biosciences, San Jose, CA, USA or Miltenyi Biotech, Bergisch Gladbach, Germany) in phosphate-buffered saline (PBS; CHUV) containing 3% fetal calf serum (FCS) for 20 min at 4 ◦C. .. After removal of unbound antibodies https://doi.org/10.3390/cells15020171 by centrifugation, cells were resuspended in 200 μL of PBS/3%FCS, and 0.5 μg of 4′,6- diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was added to identify dead cells.

    Article Title: Preserved Function of Endothelial Colony-Forming Cells in Female Rats with Intrauterine Growth Restriction: Protection Against Arterial Hypertension and Arterial Stiffness?
    Article Snippet: .. Single-cell suspensions of ECFCs from CTRL (n = 4) and IUGR (n = 4) rats were stained with fluorochrome-labeled monoclonal antibodies against CD31 PE (TLD-3A12), CD45 FITC (OX-1), and CD146 APC (LSEC) (BD Biosciences, San Jose, CA, USA or Miltenyi Biotech, Bergisch Gladbach, Germany) in phosphate-buffered saline (PBS; CHUV) containing 3% fetal calf serum (FCS) for 20 min at 4 °C. .. After removal of unbound antibodies by centrifugation, cells were resuspended in 200 μL of PBS/3%FCS, and 0.5 μg of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was added to identify dead cells.

    Bioprocessing:

    Article Title: Preserved Function of Endothelial Colony-Forming Cells in Female Rats with Intrauterine Growth Restriction: Protection Against Arterial Hypertension and Arterial Stiffness?
    Article Snippet: .. Single-cell suspensions of ECFCs from CTRL (n = 4) and IUGR (n = 4) rats were stained with fluorochrome-labeled monoclonal antibodies against CD31 PE (TLD-3A12), CD45 FITC (OX-1), and CD146 APC (LSEC) (BD Biosciences, San Jose, CA, USA or Miltenyi Biotech, Bergisch Gladbach, Germany) in phosphate-buffered saline (PBS; CHUV) containing 3% fetal calf serum (FCS) for 20 min at 4 ◦C. .. After removal of unbound antibodies https://doi.org/10.3390/cells15020171 by centrifugation, cells were resuspended in 200 μL of PBS/3%FCS, and 0.5 μg of 4′,6- diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was added to identify dead cells.

    Article Title: Preserved Function of Endothelial Colony-Forming Cells in Female Rats with Intrauterine Growth Restriction: Protection Against Arterial Hypertension and Arterial Stiffness?
    Article Snippet: .. Single-cell suspensions of ECFCs from CTRL (n = 4) and IUGR (n = 4) rats were stained with fluorochrome-labeled monoclonal antibodies against CD31 PE (TLD-3A12), CD45 FITC (OX-1), and CD146 APC (LSEC) (BD Biosciences, San Jose, CA, USA or Miltenyi Biotech, Bergisch Gladbach, Germany) in phosphate-buffered saline (PBS; CHUV) containing 3% fetal calf serum (FCS) for 20 min at 4 °C. .. After removal of unbound antibodies by centrifugation, cells were resuspended in 200 μL of PBS/3%FCS, and 0.5 μg of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was added to identify dead cells.

    Saline:

    Article Title: Preserved Function of Endothelial Colony-Forming Cells in Female Rats with Intrauterine Growth Restriction: Protection Against Arterial Hypertension and Arterial Stiffness?
    Article Snippet: .. Single-cell suspensions of ECFCs from CTRL (n = 4) and IUGR (n = 4) rats were stained with fluorochrome-labeled monoclonal antibodies against CD31 PE (TLD-3A12), CD45 FITC (OX-1), and CD146 APC (LSEC) (BD Biosciences, San Jose, CA, USA or Miltenyi Biotech, Bergisch Gladbach, Germany) in phosphate-buffered saline (PBS; CHUV) containing 3% fetal calf serum (FCS) for 20 min at 4 ◦C. .. After removal of unbound antibodies https://doi.org/10.3390/cells15020171 by centrifugation, cells were resuspended in 200 μL of PBS/3%FCS, and 0.5 μg of 4′,6- diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was added to identify dead cells.

    Article Title: Preserved Function of Endothelial Colony-Forming Cells in Female Rats with Intrauterine Growth Restriction: Protection Against Arterial Hypertension and Arterial Stiffness?
    Article Snippet: .. Single-cell suspensions of ECFCs from CTRL (n = 4) and IUGR (n = 4) rats were stained with fluorochrome-labeled monoclonal antibodies against CD31 PE (TLD-3A12), CD45 FITC (OX-1), and CD146 APC (LSEC) (BD Biosciences, San Jose, CA, USA or Miltenyi Biotech, Bergisch Gladbach, Germany) in phosphate-buffered saline (PBS; CHUV) containing 3% fetal calf serum (FCS) for 20 min at 4 °C. .. After removal of unbound antibodies by centrifugation, cells were resuspended in 200 μL of PBS/3%FCS, and 0.5 μg of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) was added to identify dead cells.



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    Leukocyte populations dynamics in domestic pigs and wild boar infected with ASFV genotype II strain “Armenia 2007”. Statistically significant differences (means) between days after infection with respect to their pre-inoculation values were evaluated using the paired t-test. Black asterisks indicate statistically significant differences. Day post-infection (x-axis); Number of cells per mL (y-axis); WBC: white blood cells (total number of <t>leukocytes);</t> TD: termination day (euthanasia was performed once the humane endpoint was reached); Variables of significance (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).
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    fitc antimouse cd45 30 f11  (Cytek Biosciences)
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    Leukocyte populations dynamics in domestic pigs and wild boar infected with ASFV genotype II strain “Armenia 2007”. Statistically significant differences (means) between days after infection with respect to their pre-inoculation values were evaluated using the paired t-test. Black asterisks indicate statistically significant differences. Day post-infection (x-axis); Number of cells per mL (y-axis); WBC: white blood cells (total number of <t>leukocytes);</t> TD: termination day (euthanasia was performed once the humane endpoint was reached); Variables of significance (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).
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    Leukocyte populations dynamics in domestic pigs and wild boar infected with ASFV genotype II strain “Armenia 2007”. Statistically significant differences (means) between days after infection with respect to their pre-inoculation values were evaluated using the paired t-test. Black asterisks indicate statistically significant differences. Day post-infection (x-axis); Number of cells per mL (y-axis); WBC: white blood cells (total number of <t>leukocytes);</t> TD: termination day (euthanasia was performed once the humane endpoint was reached); Variables of significance (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).
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    Image Search Results


    LV-FXN gene therapy does not affect the engraftment and lineage commitment of HSPCs and deposits FXN protein in FRDA-relevant tissues (A) Overview of the transplantation experiment. Lineage-negative cells isolated from LY5.1 mice were transduced with LV-FXN at a multiplicity of infection (MOI) of 20 and transplanted into lethally irradiated LY5.2 recipient mice. Three months after transplantation, hematopoietic organs were analyzed by FACS, and frataxin protein levels were measured in the spleen, brain, heart, muscle, liver, and kidney by mass spectrometry. (B–D) Percentage of CD45.1 (donor-derived) and CD45.2 (recipient-derived) cells in peripheral blood, bone marrow (BM), and spleen of mice transplanted with mock-untransduced ( n = 3) or LV-FXN-transduced cells ( n = 4) (upper), along with the lineage composition within the CD45.1 and CD45.2 compartments (lower). (E) Levels of human mature frataxin (ng per mg of total protein; mean ± SD) in the spleen of mice transplanted with mock-untransduced cells (mouse #304) or LV-FXN-transduced cells (mice #306, #307, and #310). (F) Total frataxin levels (ng per mg of total protein; mean ± SD) in the indicated organs of mice transplanted with mock-transduced cells (mouse #304) or LV-FXN- transduced cells with >1 vector copy number (VCN) (mice #307 and #310). ND = not determined.

    Journal: Cell Reports Medicine

    Article Title: Therapeutic activity of a hematopoietic stem cell-delivered cell-penetrating frataxin in Friedreich’s ataxia models

    doi: 10.1016/j.xcrm.2026.102803

    Figure Lengend Snippet: LV-FXN gene therapy does not affect the engraftment and lineage commitment of HSPCs and deposits FXN protein in FRDA-relevant tissues (A) Overview of the transplantation experiment. Lineage-negative cells isolated from LY5.1 mice were transduced with LV-FXN at a multiplicity of infection (MOI) of 20 and transplanted into lethally irradiated LY5.2 recipient mice. Three months after transplantation, hematopoietic organs were analyzed by FACS, and frataxin protein levels were measured in the spleen, brain, heart, muscle, liver, and kidney by mass spectrometry. (B–D) Percentage of CD45.1 (donor-derived) and CD45.2 (recipient-derived) cells in peripheral blood, bone marrow (BM), and spleen of mice transplanted with mock-untransduced ( n = 3) or LV-FXN-transduced cells ( n = 4) (upper), along with the lineage composition within the CD45.1 and CD45.2 compartments (lower). (E) Levels of human mature frataxin (ng per mg of total protein; mean ± SD) in the spleen of mice transplanted with mock-untransduced cells (mouse #304) or LV-FXN-transduced cells (mice #306, #307, and #310). (F) Total frataxin levels (ng per mg of total protein; mean ± SD) in the indicated organs of mice transplanted with mock-transduced cells (mouse #304) or LV-FXN- transduced cells with >1 vector copy number (VCN) (mice #307 and #310). ND = not determined.

    Article Snippet: Anti-mouse CD45.2 FITC , Miltenyi , Cat# 130-102-458; RRID:AB_2660717.

    Techniques: Transplantation Assay, Isolation, Transduction, Infection, Irradiation, Mass Spectrometry, Derivative Assay, Plasmid Preparation

    Leukocyte populations dynamics in domestic pigs and wild boar infected with ASFV genotype II strain “Armenia 2007”. Statistically significant differences (means) between days after infection with respect to their pre-inoculation values were evaluated using the paired t-test. Black asterisks indicate statistically significant differences. Day post-infection (x-axis); Number of cells per mL (y-axis); WBC: white blood cells (total number of leukocytes); TD: termination day (euthanasia was performed once the humane endpoint was reached); Variables of significance (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).

    Journal: Frontiers in Immunology

    Article Title: Dynamics of leukocyte populations, immune-regulatory cytokines, and biochemical parameters in wild boar and domestic pigs experimentally infected with a virulent African swine fever virus genotype II strain

    doi: 10.3389/fimmu.2026.1751646

    Figure Lengend Snippet: Leukocyte populations dynamics in domestic pigs and wild boar infected with ASFV genotype II strain “Armenia 2007”. Statistically significant differences (means) between days after infection with respect to their pre-inoculation values were evaluated using the paired t-test. Black asterisks indicate statistically significant differences. Day post-infection (x-axis); Number of cells per mL (y-axis); WBC: white blood cells (total number of leukocytes); TD: termination day (euthanasia was performed once the humane endpoint was reached); Variables of significance (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001).

    Article Snippet: The total number of leukocytes (WBC) and the main leukocytes subsets were evaluated by immunostaining with anti-CD45-FITC (Clone K252.1E4, Bio-Rad Antibodies, Kidlington, UK) and analyzed by flow cytometry using a MACSQuant, analyzer (Miltenyi Biotech, Bisley UK).

    Techniques: Infection